Rockefeller University Research Assistant, Human Genetics of Infectious Diseases in New York, New York
Job Title Research Assistant, Human Genetics of Infectious Diseases
Laboratory / Department Human Genetics of Infectious Diseases
The Laboratory of Human Genetics of Infectious Diseases studies the genetics of human predisposition to pediatric infectious diseases, particularly mycobacterial diseases, invasive pneumococcal diseases and herpes simplex encephalitis. It aims to identify Mendelian 'holes' in the immune defense of otherwise healthy children who are susceptible to specific infectious diseases, work that has profound implications for and has resulted in a paradigm shift in human clinical medicine and fundamental immunology. In the past decade, the laboratory has uncovered that single genetic lesions in children confer severe and selective vulnerability to certain illnesses, whereas corresponding infections in adults result more from polygenetic inheritance. This work not only blurs the distinction between patient-based Mendelian genetics and population-based complex genetics but has also provided experimental support for a unified theory of human infectious diseases.
The Research Assistant will be responsible for maintaining the induced pluripotent stem cell (iPSC) core for the laboratory. Responsibilities include culture and storage of numerous iPSC lines primarily derived from patients with diverse primary immune deficiencies, confirmation of the mutations by Sanger-sequencing of the patients' lines, and keeping track of karyotype; weekend support may be requested to feed the cells. Will provide technical support to post-docs and students for stem cell-based neuron differentiation, including medium preparation, differentiation maintenance, and neuron marker staining. The Research Assistant will maintain a growing database of the iPSC lines and provide technical assistance on relevant research projects (mainly viral encephalitis) under the supervision of an Associate Professor of the laboratory. Will also be responsible for maintaining a database of DNA samples for sequencing.
Bachelor's degree in science required; educational emphasis in biochemistry, molecular genetics, immunology, or related field preferred. Must be detail-oriented, have a strong sense of responsibility, and be able to work as part of a team in a fast-paced environment. Strong interpersonal skills, communication skills, and computer proficiency are required. Experience with tissue culture preferred.
The Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans